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Gemella massiliensis sp. nov., a new bacterium isolated from the human sputum.
- Source :
- Archives of Microbiology; Nov2021, Vol. 203 Issue 9, p5817-5823, 7p
- Publication Year :
- 2021
-
Abstract
- Thanks to its ability to isolate previously uncultured bacterial species, culturomics has dynamized the study of the human microbiota. A new bacterial species, Gemella massiliensis Marseille-P3249<superscript>T</superscript>, was isolated from a sputum sample of a healthy French man. Strain Marseille-P3249<superscript>T</superscript> is a facultative anaerobe, catalase-negative, Gram positive, coccus, and unable to sporulate. The major fatty acids were C<subscript>16:0</subscript> (34%), C<subscript>18:1n9</subscript> (28%), C<subscript>18:0</subscript> (15%) and C<subscript>18:2n6</subscript> (13%). Its 16S rRNA sequence exhibits a 98.3% sequence similarity with Gemella bergeri strain 617-93<superscript>T</superscript>, its phylogenetically closest species with standing in nomenclature. Its digital DNA–DNA hybridization (dDDH) and OrthoANI values with G. bergeri of only 59.7 ± 5.6% and 94.8%, respectively. These values are lower than the thresholds for species delineation (> 70% and > 95%, respectively). This strain grows optimally at 37 °C and its genome is 1.80 Mbp long with a 30.5 mol% G + C content. Based on these results, we propose the creation of the new species Gemella massilienis sp. nov., strain Marseille-P3249<superscript>T</superscript> (= CSUR P3249 = DSMZ 103940). [ABSTRACT FROM AUTHOR]
- Subjects :
- SPUTUM
HUMAN microbiota
FRENCH people
FATTY acids
NUCLEIC acid hybridization
Subjects
Details
- Language :
- English
- ISSN :
- 03028933
- Volume :
- 203
- Issue :
- 9
- Database :
- Complementary Index
- Journal :
- Archives of Microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 152928323
- Full Text :
- https://doi.org/10.1007/s00203-021-02493-2