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LIPUS inhibits inflammation and catabolism through the NF‐κB pathway in human degenerative nucleus pulposus cells.
- Source :
- Journal of Orthopaedic Surgery & Research; 10/18/2021, Vol. 16 Issue 1, p1-9, 9p
- Publication Year :
- 2021
-
Abstract
- Background: Low-intensity pulsed ultrasound (LIPUS) is a safe and noninvasive rehabilitative physical therapy with anti-inflammatory effects. The current study investigated the effect of LIPUS on the inflammation of nucleus pulposus (NP) cells and its underlying mechanism. Methods: Human NP cells were acquired from lumbar disc herniation tissue samples and cultured for experiments. Human NP cells were treated with LPS and then exposed to LIPUS (15 mW/cm<superscript>2</superscript>, 30 mW/cm<superscript>2</superscript> and 60 mW/cm<superscript>2</superscript>) for 20 min daily for 3 days to determine the appropriate intensity to inhibit the expression of the inflammatory factors TNF-α and IL-1β. The gene and protein expression of aggrecan, collagen II, MMP-3 and MMP-9 was measured by real‐time PCR and western blotting, respectively. The activity of the nuclear factor‐kappa B (NF‐κB) pathway was examined by western blotting and immunofluorescence. After pretreatment with the NF-κB inhibitor PDTC, the expression of TNF-α, IL-1β, MMP-3 and MMP-9 was measured by real‐time PCR. Results: LIPUS at intensities of 15 mW/cm<superscript>2</superscript>, 30 mW/cm<superscript>2</superscript> and 60 mW/cm<superscript>2</superscript> inhibited LPS-induced NP cell expression of the inflammatory factors TNF-α and IL-1β, especially at 30 mW/cm<superscript>2</superscript>. LIPUS significantly upregulated the gene and protein expression of aggrecan and collagen II and downregulated the gene and protein expression of MMP-3 and MMP-9 in LPS-induced NP cells. The NF‐κB signaling pathway was inhibited by LIPUS through inhibiting the protein expression of p-P65 and the translocation of P65 into the nucleus in LPS-induced NP cells. In addition, LIPUS had similar effects as the NF-κB inhibitor PDTC by inhibiting the NF-κB signaling pathway, inflammation and catabolism in LPS-induced human degenerative nucleus pulposus cells. Conclusion: LIPUS inhibited inflammation and catabolism through the NF‐κB pathway in human degenerative nucleus pulposus cells. [ABSTRACT FROM AUTHOR]
- Subjects :
- INTERLEUKINS
REVERSE transcriptase polymerase chain reaction
CYTOKINES
WESTERN immunoblotting
INFLAMMATION
METABOLISM
GENE expression
MATRIX metalloproteinases
DNA-binding proteins
TUMOR necrosis factors
FLUORESCENT antibody technique
DISCITIS
CELL lines
POLYMERASE chain reaction
LUMBAR vertebrae
ULTRASONICS
Subjects
Details
- Language :
- English
- ISSN :
- 1749799X
- Volume :
- 16
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- Journal of Orthopaedic Surgery & Research
- Publication Type :
- Academic Journal
- Accession number :
- 153076988
- Full Text :
- https://doi.org/10.1186/s13018-021-02739-1