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Hepatoprotective Effect and Potential Mechanism of Aqueous Extract from Phyllanthus emblica on Carbon-Tetrachloride-Induced Liver Fibrosis in Rats.
- Source :
- Evidence-based Complementary & Alternative Medicine (eCAM); 10/19/2021, p1-12, 12p, 3 Color Photographs, 1 Diagram, 2 Charts, 5 Graphs
- Publication Year :
- 2021
-
Abstract
- Liver fibrosis is a pathological variation caused by almost all chronic liver injuries. As an edible and medicinal natural resource, Phyllanthus emblica (PE) has been reported to possess hepatoprotective, antioxidant, and anti-inflammatory activities and may have an ameliorating effect on hepatic fibrosis. To investigate the protective effect of the aqueous extract of PE (AEPE) against liver fibrosis and to uncover its related mechanisms, the chemical profile of AEPE was characterized by high performance liquid chromatography (HPLC) and sulfuric acid-phenol method. Ameliorative effects of different doses of AEPE were investigated in carbon-tetrachloride- (CCl<subscript>4</subscript>-) induced liver fibrosis rats by analyzing biochemical markers, morphologic pathology, and related proteins expression in liver tissue. The results indicated that AEPE (1.8, 3.6 g/kg) could significantly reduce levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), collagen IV (Col IV), type III precollagen (PCIII), hyaluronic acid (HA), laminin (LN), malondialdehyde (MDA), nitric oxide (NO), protein carbonyl (PC), tumor necrosis factor-α(TNF-α), interleukin-1β (IL-1β), interleukin-6 (IL-6), and hydroxyproline (Hyp) and increase the levels of superoxide dismutase (SOD), glutathione (GSH), and catalase (CAT). Hematoxylin-eosin (H&E), Sirius red, and Masson staining showed AEPE-treated improved fibrotic lesions and inflammatory cell infiltration. Meanwhile, AEPE treatment also significantly downregulates the expression of α-smooth muscle actin (α-SMA) and transforming growth factor-β1 (TGF-β1) in the liver tissue and serum, respectively. In conclusion, AEPE possesses curative efficacy against liver fibrosis through its antioxidant, anti-inflammatory, and antifibrotic effects. [ABSTRACT FROM AUTHOR]
- Subjects :
- BIOMARKERS
PROTEINS
COLLAGEN
INTERLEUKINS
GLUTATHIONE
MEDICINAL plants
PHENOLS
ANIMAL experimentation
LIQUID chromatography
FIBROSIS
INTERLEUKIN-1
SUPEROXIDE dismutase
HEPATOTOXICOLOGY
HYDROCARBONS
LIVER diseases
RATS
GENE expression
HYALURONIC acid
MALONDIALDEHYDE
OXIDATIVE stress
CATALASE
TUMOR necrosis factors
PROLINE
BENZOPYRANS
PLANT extracts
MOLECULAR structure
SULFURIC acid
MEMBRANE proteins
NITRIC oxide
FLUORESCENT dyes
ALANINE aminotransferase
ASPARTATE aminotransferase
Subjects
Details
- Language :
- English
- ISSN :
- 1741427X
- Database :
- Complementary Index
- Journal :
- Evidence-based Complementary & Alternative Medicine (eCAM)
- Publication Type :
- Academic Journal
- Accession number :
- 153094294
- Full Text :
- https://doi.org/10.1155/2021/5345821