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Stable Super‐Resolution Imaging of Lipid Droplet Dynamics through a Buffer Strategy with a Hydrogen‐Bond Sensitive Fluorogenic Probe.
- Source :
- Angewandte Chemie; 11/15/2021, Vol. 133 Issue 47, p25308-25317, 10p
- Publication Year :
- 2021
-
Abstract
- Although super‐resolution imaging offers an opportunity to visualize cellular structures and organelles at the nanoscale level, cellular heterogeneity and unpredictability still pose a significant challenge in the dynamic imaging of live cells. It is thus vital to develop better‐performing and more photostable probes for long‐term super‐resolution imaging. Herein, we report a probe, LD‐FG, for imaging lipid droplet (LD) dynamics using structured illumination microscopy (SIM). LD‐FG allows wash‐free imaging of LDs, owing to a hydrogen‐bond sensitive fluorogenic response. The replacement of photobleached LD‐FG by intact probe molecules outside the LDs ensures the long‐time stability of the fluorescence imaging. With this buffering fluorogenic probe, fast and unpredictable dynamic processes of LDs can be visualized. Using this probe, two LD coalescence modes were discovered. The dynamic imaging also allowed us to propose a new model of LD maturation during adipocyte differentiation, i.e. a fast LD coalescence followed by a slow ripening step. The excellent performance of LD‐FG makes the buffer strategy an effective method for designing fluorescent probes for cell dynamic imaging. [ABSTRACT FROM AUTHOR]
- Subjects :
- HIGH resolution imaging
FLUORESCENT probes
LIPIDS
CELL anatomy
CELL imaging
FAT cells
Subjects
Details
- Language :
- English
- ISSN :
- 00448249
- Volume :
- 133
- Issue :
- 47
- Database :
- Complementary Index
- Journal :
- Angewandte Chemie
- Publication Type :
- Academic Journal
- Accession number :
- 153458384
- Full Text :
- https://doi.org/10.1002/ange.202111052