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Super-assembled silica nanoprobes for intracellular Zn(II) sensing and reperfusion injury treatment through in situ MOF crystallization.
- Source :
- Analyst; 11/21/2021, Vol. 146 Issue 22, p6788-6797, 10p
- Publication Year :
- 2021
-
Abstract
- The production of excess free zinc ions (Zn<superscript>2+</superscript>) in cells has been identified as an important cause of cell injury or apoptosis after ischemia reperfusion. Thus, developing a nanosystem with multiple therapeutic functions to significantly eliminate multiple cell injury factors is of great interest. Here, a super-assembled nanosystem consisting of a polyethylene glycol (PEG) surface-modified mesoporous silica nanoparticle (MSN) encapsulating 2-methylimidazole (2MI) and a Zn<superscript>2+</superscript> probe (PZn) was fabricated. The 2MI-P@MSN nanoassemblies showed a "turn-on" fluorescence signal at 476 nm toward zinc ions due to the presence of PZn. Besides, zeolitic imidazolate framework-8 (ZIF-8) could be assembled on the site intracellularly after 2MI chelating with free zinc ions. The experimental results revealed that 2MI-P@MSN exhibited excellent biocompatibility and non-cytotoxicity, and was able to provide satisfactory protection to OGD/R-treated cells based on zinc ion adsorption and the antioxidant effect of ZIF-8, which could effectively improve the survival rate of reperfusion injury cells from 52% to 73%. Notably, selective and quantitative sensing of Zn<superscript>2+</superscript> was successfully carried out in the cells. This strategy highlights the potential of the detection, absorption and assembly of excess zinc ions simultaneously for cell therapy, which provides a promising therapeutic method for ischemic stroke, oxidative damage and diseases associated with zinc ion accumulation. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00032654
- Volume :
- 146
- Issue :
- 22
- Database :
- Complementary Index
- Journal :
- Analyst
- Publication Type :
- Academic Journal
- Accession number :
- 153460958
- Full Text :
- https://doi.org/10.1039/d1an01475g