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Gold-based immunochromatographic assay strip for the detection of quinclorac in foods.
- Source :
- Analyst; 11/21/2021, Vol. 146 Issue 22, p6831-6839, 9p
- Publication Year :
- 2021
-
Abstract
- In this study, a highly specific and sensitive monoclonal antibody (mAb) against quinclorac (Qui) was prepared. Based on the selected mAb, 2G3, an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and an immunochromatographic strip assay were established for the detection of Qui in actual samples. The 50%-inhibitory concentration of mAb 2G3 against Qui was 48.763 ng mL<superscript>−1</superscript>. No cross-reaction with other quinolines indicated that mAb 2G3 had high specificity. The recovery of the established ic-ELISA method was in the range of 85.6% to 98.9%. The cut-off value of Qui in cucumber and tomato by immunochromatographic strip was 200 ng g<superscript>−1</superscript>. The analysis results of ic-ELISA and immunochromatographic strip assay were consistent with the results of LC-MS/MS, which further proved that the established ic-ELISA and immunochromatographic strip assay could provide valuable tools for the rapid detection of Qui residues in cucumber and tomato samples. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00032654
- Volume :
- 146
- Issue :
- 22
- Database :
- Complementary Index
- Journal :
- Analyst
- Publication Type :
- Academic Journal
- Accession number :
- 153460968
- Full Text :
- https://doi.org/10.1039/d1an01748a