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IGF-1 treatment during in vitro maturation improves developmental potential of ovine oocytes through the regulation of PI3K/Akt and apoptosis signaling.
- Source :
- Animal Biotechnology; Dec 2021, Vol. 32 Issue 6, p798-805, 8p
- Publication Year :
- 2021
-
Abstract
- This study aimed to assess the effect of the insulin-like grow factor 1 (IGF-1) treatment during in vitro maturation on the gene expression and developmental ability of ovine oocytes. Ovine cumulus-oocyte complexes (COC) were matured in vitro without (control) or with the supplementation of IGF-1 (100 ng/ml) and then subjected to in vitro fertilization and culture. The rate of oocyte maturation and embryo development was recorded and expression of the selected genes (involved in the PI3K/Akt and apoptosis signaling) was assessed in the matured oocytes. The IGF-1 treatment significantly (p <.05) improved the oocyte maturation rate (%) as compared to the control (81.5 ± 2.40 vs. 73.6 ± 0.94). Similarly, as compared to the control, the IGF-1 treatment significantly (p <.05) improved the rate (%) of cleavage (54.7 ± 1.58 vs. 67.2 ± 3.65) and the formation of 4–8 cell embryos (30.7 ± 2.89 vs. 44.1 ± 4.01) and morula (20.7 ± 2.08 vs. 32.8 ± 2.78). The IGF-1 treatment significantly (p <.05) upregulated the expression of IGF1R, PI3KR1, AKT1 and BCL2 and downregulated the expression of GSK3β, FOXO3 and CASP9 in the matured oocytes. In conclusion, the IGF-1 treatment significantly improved the developmental competence of ovine oocytes through the regulation of the PI3K/Akt and apoptosis signaling. [ABSTRACT FROM AUTHOR]
- Subjects :
- PI3K/AKT pathway
OVUM
FERTILIZATION in vitro
GENE expression
EMBRYOS
Subjects
Details
- Language :
- English
- ISSN :
- 10495398
- Volume :
- 32
- Issue :
- 6
- Database :
- Complementary Index
- Journal :
- Animal Biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 154077708
- Full Text :
- https://doi.org/10.1080/10495398.2020.1752703