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Microparticle-tagged image-based cell counting (ImmunoSpin) for CD4 + T cells.
- Source :
- Microchimica Acta; Dec2021, Vol. 188 Issue 12, p1-10, 10p
- Publication Year :
- 2021
-
Abstract
- Affordable point-of-care (POC) CD4 + T lymphocyte counting techniques have been developed as alternatives to flow cytometry-based instruments caring for patients with human immunodeficiency virus (HIV)-1. However, POC CD4 enumeration technologies can be inaccurate. Here, we developed a microparticle-based visual detector of CD4 + T lymphocytes (ImmunoSpin) using microparticles conjugated with anti-CD4 antibodies, independent of microfluidic or fluorescence detection systems. Visual enumeration of CD4 + T cells under conventional light microscope was accurate compared to flow cytometry. Microparticle-tagged CD4 + T cells were well-recognized under a light microscope. ImmunoSpin showed very good precision (coefficients of variation of ImmunoSpin were ≤ 10%) and high correlation with clinical-grade flow cytometry for the enumeration of CD4 + T cells (y = 0.4232 + 0.9485 × for the %CD4 + T cell count, R<superscript>2</superscript> = 0.99). At thresholds of 200 and 350 cells/µL, there was no misclassification of the ImmunoSpin system compared to the reference flow cytometry. ImmunoSpin showed clear differential classification of CD4 + T lymphocytes from granulocytes and monocytes. Because non-fluorescence microparticle-tags and cytospin slides are used in ImmunoSpin, they can be applied to an automatic digital image analyzer. Slide preparation allows long-term storage, no analysis time limitations, and image transfer in remote areas. [ABSTRACT FROM AUTHOR]
- Subjects :
- T cells
CD4 antigen
LYMPHOCYTE count
HIV
MICROSCOPES
FLOW cytometry
Subjects
Details
- Language :
- English
- ISSN :
- 00263672
- Volume :
- 188
- Issue :
- 12
- Database :
- Complementary Index
- Journal :
- Microchimica Acta
- Publication Type :
- Academic Journal
- Accession number :
- 154212997
- Full Text :
- https://doi.org/10.1007/s00604-021-05070-y