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A colloidal gold immunochromatographic strip assay for the rapid detection of Shigella in milk and meat products.

Authors :
Ang, Beijun
Xu, Xinxin
Liu, Liqiang
Xu, Liguang
Kuang, Hua
Xu, Chuanlai
Source :
New Journal of Chemistry; 1/7/2022, Vol. 46 Issue 1, p103-109, 7p
Publication Year :
2022

Abstract

Shigella is the most common foodborne pathogen and can cause bacillary dysentery. Therefore, it is necessary to detect Shigella rapidly and accurately, but it is difficult for traditional methods to meet these requirements. In this study, monoclonal antibodies (mAbs) were prepared using a recombinant invasive plasmid antigen C (IpaC) protein, which is specifically located on the outer membrane of Shigella. Based on our mAbs 2G11 and 5A11, a colloidal gold lateral flow immunochromatographic (LFIA) test strip method for the detection of several serotypes of Shigella was produced. The sensitivity of the LFIA strip was 1 × 10<superscript>5</superscript> CFU mL<superscript>−1</superscript> for four groups, and seven serotypes of Shigella. The applicability of the strip was tested using spiked milk and meat samples and the results showed that Shigella could be detected in these matrices. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
11440546
Volume :
46
Issue :
1
Database :
Complementary Index
Journal :
New Journal of Chemistry
Publication Type :
Academic Journal
Accession number :
154217109
Full Text :
https://doi.org/10.1039/d1nj04708f