Back to Search
Start Over
Neglectibacter timonensis gen. nov., sp. nov. and Scatolibacter rhodanostii gen. nov., sp. nov., two anaerobic bacteria isolated from human stool samples.
- Source :
- Archives of Microbiology; Jan2022, Vol. 204 Issue 1, p1-11, 11p
- Publication Year :
- 2022
-
Abstract
- Strains Marseille-P2265<superscript>T</superscript> (=CSUR P2265<superscript>T</superscript> =DSM 102082<superscript> T</superscript>) and Marseille-P3890<superscript>T</superscript> (=CSUR P3890<superscript>T</superscript> =CCUG 72341<superscript> T</superscript>) were isolated from stool samples using the culturomics approach. The 16S rRNA gene sequences of both strains were sequenced and compared by BLASTn to the NCBI database. Strains Marseille-P2265<superscript>T</superscript> and Marseille-P3890<superscript>T</superscript> were most closely related to Acutalibacter muris with identities of 94.3% and 91.5%, respectively. Between the two strains, the 16S rRNA gene sequence identity was 91.5%. Both strains are anaerobic Gram-positive, oxidase- and catalase-negative. The major fatty acid methyl esters (> 10%) in both strains are C<subscript>16:0</subscript> and anteiso-C<subscript>15:0</subscript>. Additionally, strain Marseille-P2265<superscript>T</superscript> has iso-C<subscript>15:0</subscript> and C<subscript>14:0</subscript>, and strain Marseille-P3890<superscript>T</superscript>, iso-C<subscript>14:0</subscript>. Strain Marseille-P2265<superscript>T</superscript> has a genome size of 3,671,396-bp with a G + C content of 52.8%. As for strain Marseille-P3890<superscript>T</superscript>, the genome is 2,702,024-bp-long with a 39.8% G + C content. The genomic comparison of closely related species with strains Marseille-P2265<superscript>T</superscript> and Marseille-P3890<superscript>T</superscript> showed that all digital DNA-DNA hybridization (dDDH), orthologous average nucleotide identity (OrthoANI) and average amino acid identity (AAI) values were lower than the published species thresholds (70% for dDDH, 95–96% for OrthoANI/AAI). Based on these results, it was concluded that strains Marseille-P2265<superscript>T</superscript> and Marseille-P3890<superscript>T</superscript> belong to two new genera in the family Oscillospiraceae. For these two genera, the names Neglectibacter gen. nov. and Scatolibacter gen. nov. were proposed, with strains Marseille-P2265<superscript>T</superscript> and Marseille-P3890<superscript>T</superscript> being the type strains of Neglectibacter timonensis gen. nov., sp. nov. and Scatolibacter rhodanostii gen. nov., sp. nov., respectively. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 03028933
- Volume :
- 204
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- Archives of Microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 154278933
- Full Text :
- https://doi.org/10.1007/s00203-021-02712-w