Tandem Mass Tag-Based Proteomics Reveals the effect of Electron Beam Irradiation on Metabolism-Related Differentially Expressed Proteins in Solenocera melantho Postmortem.

Postmortem metabolism in shrimp is an important factor affecting muscle quality during its preservation. Electron beam irradiation (EBI) can effectively kill microorganisms and inactivate enzymes. In this study, the tandem mass tag (TMT) method was used to study the changes in the muscle metabolism of Solenocera melantho postmortem before and after EBI (6 kGy). The TMT method identified a total of 656 proteins, including 120 differentially expressed proteins (DEPs) (56 upregulated, 64 downregulated). The analysis of these results showed that EBI can inactivate most enzymes and slow down their corresponding metabolic processes. These results also showed that irradiation can denature some proteins. The study revealed the effects of EBI on the muscle quality of S. melantho at the molecular level. [ABSTRACT FROM AUTHOR]