Back to Search
Start Over
A microfluidic cathodic photoelectrochemical biosensor chip for the targeted detection of cytokeratin 19 fragments 21-1.
- Source :
- Lab on a Chip; 1/14/2021, Vol. 21 Issue 2, p378-384, 7p
- Publication Year :
- 2021
-
Abstract
- A microfluidic chip integrated with a microelectrode and a cathodic photoelectrochemical (PEC) biosensor for the ultrasensitive detection of non-small cell lung cancer cytokeratin fragments based on a signal amplification strategy was designed. The mechanism for signal amplification is developed based on the p–n junction of AgI/Bi<subscript>2</subscript>Ga<subscript>4</subscript>O<subscript>9</subscript>, with dissolved O<subscript>2</subscript> as an electron acceptor to produce the superoxide anion radical (˙O<subscript>2</subscript><superscript>−</superscript>) as the working microelectrode. By combining this with a novel superoxide-dismutase-loaded honeycomb manganese oxide nanostructure (SOD@hMnO<subscript>2</subscript>) as the co-catalyst signal amplification label, ˙O<subscript>2</subscript><superscript>−</superscript> can be catalyzed by SOD via a disproportionation reaction to produce O<subscript>2</subscript> and H<subscript>2</subscript>O<subscript>2</subscript>; then, hMnO<subscript>2</subscript> is able to trigger the decomposition of H<subscript>2</subscript>O<subscript>2</subscript> to generate O<subscript>2</subscript> and H<subscript>2</subscript>O. Therefore, the increased O<subscript>2</subscript> promotes the separation of electron–hole pairs via consuming more electrons, leading to an effective enhancement of the cathodic PEC behavior. Under optimum conditions, with the cytokeratin 19 fragments 21-1 (CYFRA 21-1) as the targeted detection objects, the microfluidic cathodic PEC biosensor chip exhibited excellent linearity from 0.1 pg mL<superscript>−1</superscript> to 100 ng mL<superscript>−1</superscript>, with a detection limit of 0.026 pg mL<superscript>−1</superscript> (S/N = 3). The exciting thing that this work offers is a new strategy for the detection of other important cancer biomarkers for disease diagnosis and prognosis. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 14730197
- Volume :
- 21
- Issue :
- 2
- Database :
- Complementary Index
- Journal :
- Lab on a Chip
- Publication Type :
- Academic Journal
- Accession number :
- 154435732
- Full Text :
- https://doi.org/10.1039/d0lc01063d