A look at the precision, sensitivity and specificity of SARS-CoV-2 RT-PCR assays through a dedicated external quality assessment round.

The differences in C SB t sb values reported for each individual gene target were calculated per participant as [S6 C SB t sb value - S2 C SB t sb value] and ranged from -5.3 to +6.5 cycles. Keywords: Ct value; precision; RT-PCR; SARS-CoV-2; sensitivity; specificity EN Ct value precision RT-PCR SARS-CoV-2 sensitivity specificity e34 e37 4 01/07/22 20220115 NES 220115 To the Editor, In an external quality assessment (EQA) round for SARS-CoV-2 virus genome detection in early 2021, we found first indications of the precision of individual SARS-CoV-2 RT-PCR assays in the linearity of the C SB t sb values obtained for three samples from a dilution series (1:1, 1:10, 1:100) [[1]]. Seven assays reported a C SB t sb value for one of the target genes they used for only one of samples S2 and S6 and none for the second. [Extracted from the article]