Asialoglycoprotein Receptor Targeted Magnetic Nanoparticles For Hepatocellular Carcinoma Treatment.

Objective: Hepatocellular carcinoma is the most common type of liver cancer. Doxorubicin (Dox) is an anthracycline antibiotic and is commonly used for cancer treatment. However, Dox has severe side effects that limit efficiency, and it is used with drug delivery systems. Asialoglycoprotein receptors are membrane glycoprotein receptors and are expressed in hepatocellular carcinoma. This study, it is aimed to develop a magnetic field and asialoglycoprotein receptor-targeted, doxorubicin-loaded nanoparticle system to be used in the treatment of hepatocellular carcinoma. Research methods: Magnetic nanoparticles were synthesized by the co-precipitation method. The surface was functionalized with 2-aminoethylphosphonic acid to provide the binding of arabinogalactan with magnetic nanoparticles. Then, MNP's surface was coated with arabinogalactan. After that, doxorubicin was loaded on arabinogalactan-coated magnetic nanoparticles by adsorption. The functional groups, hydrodynamic size, zeta potential, and drug encapsulation efficiency were evaluated. Results and Conclusion: The SEM size of the Dox-loaded arabinogalactan-coated magnetic nanoparticle(DANP) was found to be 30-35 nm. FTIR analysis has confirmed the presence of doxorubicin on arabinogalactan-coated ANPs. Dox loading efficiency was determined as 78.54 %. Based on the results obtained, the study is thought to have potential for in vitro, in vivo, and ex vivo studies. [ABSTRACT FROM AUTHOR]