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A single-cell atlas of mouse lung development.
- Source :
- Development (09501991); 12/15/2021, Vol. 148 Issue 24, p1-15, 15p
- Publication Year :
- 2021
-
Abstract
- Lung organogenesis requires precise timing and coordination to effect spatial organization and function of the parenchymal cells. To provide a systematic broad-based view of the mechanisms governing the dynamic alterations in parenchymal cells over crucial periods of development, we performed a single-cell RNA-sequencing timeseries yielding 102,571 epithelial, endothelial and mesenchymal cells across nine time points from embryonic day 12 to postnatal day 14 in mice. Combining computational fate-likelihood prediction with RNA in situ hybridization and immunofluorescence, we explore lineage relationships during the saccular to alveolar stage transition. The utility of this publicly searchable atlas resource (www.sucrelab.org/lungcells) is exemplified by discoveries of the complexity of type 1 pneumocyte function and characterization of mesenchymal Wnt expression patterns during the saccular and alveolar stages - wherein major expansion of the gas-exchange surface occurs. We provide an integrated view of cellular dynamics in epithelial, endothelial and mesenchymal cell populations during lung organogenesis. [ABSTRACT FROM AUTHOR]
- Subjects :
- LUNG development
CELL populations
ENDOTHELIAL cells
IN situ hybridization
MICE
LUNGS
Subjects
Details
- Language :
- English
- ISSN :
- 09501991
- Volume :
- 148
- Issue :
- 24
- Database :
- Complementary Index
- Journal :
- Development (09501991)
- Publication Type :
- Academic Journal
- Accession number :
- 154635999
- Full Text :
- https://doi.org/10.1242/dev.199512