Back to Search
Start Over
PDGFRα reporter activity identifies periosteal progenitor cells critical for bone formation and fracture repair.
- Source :
- Bone Research; 1/25/2022, Vol. 10 Issue 1, p1-15, 15p
- Publication Year :
- 2022
-
Abstract
- The outer coverings of the skeleton, which is also known as the periosteum, are arranged in concentric layers and act as a reservoir for tissue-specific bone progenitors. The cellular heterogeneity within this tissue depot is being increasingly recognized. Here, inducible PDGFRα reporter animals were found to mark a population of cells within the periosteum that act as a stem cell reservoir for periosteal appositional bone formation and fracture repair. During these processes, PDGFRα reporter<superscript>+</superscript> progenitors give rise to Nestin<superscript>+</superscript> periosteal cells before becoming osteoblasts and osteocytes. The diphtheria toxin-mediated ablation of PDGFRα reporter<superscript>+</superscript> cells led to deficits in cortical bone formation during homeostasis and a diminutive hard callus during fracture repair. After ossicle transplantation, both mouse PDGFRα reporter<superscript>+</superscript> periosteal cells and human Pdgfrα<superscript>+</superscript> periosteal progenitors expand, ossify, and recruit marrow to a greater extent than their counterpart periosteal cells, whereas PDGFRα reporter<superscript>−</superscript> periosteal cells exhibit a predisposition to chondrogenesis in vitro. Total RNA sequencing identified enrichment of the secreted factors Fermt3 and Ptpn6 within PDGFRα reporter<superscript>+</superscript> periosteal cells, which partly underlie the osteoblastogenic features of this cell population. [ABSTRACT FROM AUTHOR]
- Subjects :
- PERIOSTEUM
BONE growth
PROGENITOR cells
BONE fractures
BONE cells
COMPACT bone
Subjects
Details
- Language :
- English
- ISSN :
- 20954700
- Volume :
- 10
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- Bone Research
- Publication Type :
- Academic Journal
- Accession number :
- 154873051
- Full Text :
- https://doi.org/10.1038/s41413-021-00176-8