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Development of effective biotransformation process for benzohydroxamic acid production using Bacillus smithii IIIMB2907.
- Source :
- 3 Biotech; 2/9/2022, Vol. 12 Issue 2, p1-14, 14p
- Publication Year :
- 2022
-
Abstract
- The present study entails the usefulness of thermophilic amidase-producing bacterium in the biotransformation of benzamide to benzohydroxamic acid (BHA). A bacterium Bacillus smithii IIIMB2907 was isolated from a soil sample collected from hot springs of Manikaran, Himachal Pradesh, India. The whole cells of the bacterium displayed versatile substrate specificity by exhibiting significant activity with a diverse range of amides. In addition, amidase from thermophilic bacterium was induced by adding Ɛ-caprolactam in the mineral base media. The optimum temperature and pH of acyltransferase activity of amidase enzyme were found to be 50 °C and 7.0, respectively. Interestingly, half-life (t<subscript>1/2</subscript>) of this enzyme was 17.37 h at 50 °C. Bench-scale production and purification of BHA was carried out at optimized conditions which resulted in the recovery of 64% BHA with a purity of 96%. Owing to this, the reported process in the present study can be considered of immense industrial significance for the production of BHA. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 2190572X
- Volume :
- 12
- Issue :
- 2
- Database :
- Complementary Index
- Journal :
- 3 Biotech
- Publication Type :
- Academic Journal
- Accession number :
- 155184172
- Full Text :
- https://doi.org/10.1007/s13205-022-03109-2