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A common F-box gene regulates the leucine homeostasis of Medicago truncatula and Arabidopsis thaliana.
- Source :
- Protoplasma; Mar2022, Vol. 259 Issue 2, p277-290, 14p
- Publication Year :
- 2022
-
Abstract
- The F-box domain is a conserved structural protein motif that most frequently interacts with the SKP1 protein, the core of the SCFs (SKP1-CULLIN-F-box protein ligase) E3 ubiquitin protein ligases. As part of the SCF complexes, the various F-box proteins recruit substrates for degradation through ubiquitination. In this study, we functionally characterized an F-box gene (MtF-box) identified earlier in a population of Tnt1 retrotransposon-tagged mutants of Medicago truncatula and its Arabidopsis thaliana homolog (AtF-box) using gain- and loss-of-function plants. We highlighted the importance of MtF-box in leaf development of M. truncatula. Protein–protein interaction analyses revealed the 2-isopropylmalate synthase (IPMS) protein as a common interactor partner of MtF-box and AtF-box, being a key enzyme in the biosynthesis pathway of the branched-chain amino acid leucine. For further detailed analysis, we focused on AtF-box and its role during the cell division cycle. Based on this work, we suggest a mechanism for the role of the studied F-box gene in regulation of leucine homeostasis, which is important for growth. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 0033183X
- Volume :
- 259
- Issue :
- 2
- Database :
- Complementary Index
- Journal :
- Protoplasma
- Publication Type :
- Academic Journal
- Accession number :
- 155380951
- Full Text :
- https://doi.org/10.1007/s00709-021-01662-w