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Non-Destructive Monitoring via Electrochemical NADH Detection in Murine Cells.
- Source :
- Biosensors (2079-6374); Feb2022, Vol. 12 Issue 2, p107, 1p
- Publication Year :
- 2022
-
Abstract
- Nicotinamide adenine dinucleotide (NADH) is an important cofactor involved in metabolic redox reactions in living cells. The detection of NADH in living animal cells is a challenge. We developed a one-step monitoring method for NADH via an electrocatalytic reaction that uses a surface-modified, screen-printed electrode (SPE) having a redox active monolayer 4′-mercapto-N-phenlyquinone diamine (NPQD) formed by a self-assembled monolayer (SAM) of an aromatic thiol, 4-aminothiophenol (4-ATP). This electrode has a limit of detection (LOD) of 0.49 μM and a sensitivity of 0.0076 ± 0.0006 μM/μA in cell culture media, which indicates that it retains its selectivity. The applicability of this NADH sensor was demonstrated for the first time by cell viability monitoring via NADH-sensing in cell culture supernatants. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 20796374
- Volume :
- 12
- Issue :
- 2
- Database :
- Complementary Index
- Journal :
- Biosensors (2079-6374)
- Publication Type :
- Academic Journal
- Accession number :
- 155497545
- Full Text :
- https://doi.org/10.3390/bios12020107