Aged and young mice differentially respond to tape‐stripping in epidermal gene expression.

Disruption of epidermal permeability barrier induces an increase in proinflammatory cytokine expression and release, stimulation of epidermal lipid and DNA synthesis, and expression of antimicrobial peptides. Although alterations in epidermal function in the aged skin are known, whether the epidermal transcriptomic responses to barrier disruption differ between aged and young mice remains unknown. Here, we performed RNA sequencing of the epidermis in 2‐month‐ vs. 20‐month‐old mice following barrier disruption with repeated tape‐stripping. At baseline condition, the epidermis of 20‐month‐old mice displayed an upregulation of inflammation‐associated genes and down‐regulation of epidermal structure‐ and development‐related genes in comparison to 2‐month‐old mice. Barrier disruption upregulated expression levels of 327 genes and downregulated 209 genes in 2‐month‐old mice. In 20‐month‐old mice, the numbers of upregulated and down‐regulated genes were 537 and 299, respectively. In comparison to young mice, the prominently upregulated genes in the 20‐month‐old mice were associated with the IL‐17 signalling pathway, while downregulated genes were mainly involved in the cytokine–cytokine receptor interaction pathway. These results indicate that inflammation‐associated signalling pathways are upregulated, while epidermal structure‐ and development‐related genes are downregulated in the epidermis of aged mice, with further aggravation following barrier disruption, suggesting the importance of improving epidermal function in the elderly. [ABSTRACT FROM AUTHOR]