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Distinct spatial arrangements of ACE2 and TMPRSS2 expression in Syrian hamster lung lobes dictates SARS-CoV-2 infection patterns.
- Source :
- PLoS Pathogens; 3/7/2022, Vol. 18 Issue 3, p1-23, 23p
- Publication Year :
- 2022
-
Abstract
- SARS-CoV-2 attaches to angiotensin-converting enzyme 2 (ACE2) to gain entry into cells after which the spike protein is cleaved by the transmembrane serine protease 2 (TMPRSS2) to facilitate viral-host membrane fusion. ACE2 and TMPRSS2 expression profiles have been analyzed at the genomic, transcriptomic, and single-cell RNAseq levels. However, transcriptomic data and actual protein validation convey conflicting information regarding the distribution of the biologically relevant protein receptor in whole tissues. To describe the organ-level architecture of receptor expression, related to the ability of ACE2 and TMPRSS2 to mediate infectivity, we performed a volumetric analysis of whole Syrian hamster lung lobes. Lung tissue of infected and control animals was stained using antibodies against ACE2 and TMPRSS2, combined with SARS-CoV-2 nucleoprotein staining. This was followed by light-sheet microscopy imaging to visualize their expression and related infection patterns. The data demonstrate that infection is restricted to sites containing both ACE2 and TMPRSS2, the latter is expressed in the primary and secondary bronchi whereas ACE2 is predominantly observed in the bronchioles and alveoli. Conversely, infection completely overlaps where ACE2 and TMPRSS2 co-localize in the tertiary bronchi, bronchioles, and alveoli. Author summary: The ongoing COVID19 pandemic necessitates additional tools to study SARS-CoV infection dynamics. This increases our understanding of where these viruses cause infections in the lung, and how essential different receptor molecules are. Here we applied tissue optical clearing and 3D immunofluorescence of whole organs from our best animal model, the Syrian hamster. We describe the organ-level architecture of receptor expression profiles, to visualize how their concerted action mediates SARS-CoV-2 infection patterns. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 15537366
- Volume :
- 18
- Issue :
- 3
- Database :
- Complementary Index
- Journal :
- PLoS Pathogens
- Publication Type :
- Academic Journal
- Accession number :
- 155606013
- Full Text :
- https://doi.org/10.1371/journal.ppat.1010340