Identification of miRNAs and their target genes in response to salt stress in Brassica napus.

Brassica napus L. is one of the important oil crops in the world. However soil salt stress severely inhibited its growth and development. Previous studies have shown that under salt stress, plants increase their own salt stress resistance by expressing some miRNAs and regulating the expression of their target genes. In this study, rapeseed was cultured under 200 mmol⋅L^-1 NaCl treatment and control, and took the shoot and root to construct 12 small RNA libraries for high-throughput sequencing. Through the differential expression analysis of miRNAs in the whole genome, a total of 26 differentially expressed miRNAs were identified and 171 corresponding target genes were predicted. Combining the differential expression of genes in the early transcriptome and the results of the differential expression of miRNAs in this study, it is speculated that miRNA156-SPL15-WRKY, miRNA397-LAC12-xylogen, miR169-NFYA5 and miR399-UBC29-ubiquitination pathways might be involved in the regulation of salt stress resistance in B. napus. [ABSTRACT FROM AUTHOR]