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Attenuating ischemia-reperfusion injury with polymerized albumin.

Authors :
Belcher, Donald A.
Williams, Alexander T.
Munoz, Carlos J.
Muller, Cynthia R.
Walser, Cynthia
Palmer, Andre F.
Cabrales, Pedro
Source :
Journal of Applied Physiology; Feb2022, Vol. 132 Issue 2, p489-496, 8p
Publication Year :
2022

Abstract

Ischemia-reperfusion injury increased vascular permeability, resulting in fluid extravasation from the intravascular compartment into the tissue space. Fluid and small protein extravasation lead to increased interstitial fluid pressure and capillary collapse, impairing capillary exchange. Polymerized human serum albumin (PolyHSA) has an increased molecular weight (MW) compared with unpolymerized human serum albumin (HSA) and can improve intravascular fluid retention and recovery from ischemia-reperfusion injury. To test the hypothesis that polymerization of HSA can improve recovery from ischemia-reperfusion injury, we studied how exchange transfusion of 20% of the blood volume with HSA or PolyHSA immediately before reperfusion can affect local ischemic tissue microhemodynamics, vascular integrity, and tissue viability in a hamster dorsal window chamber model. Microvascular flow and functional capillary density were maintained in animals exchanged with PolyHSA compared with HSA. Likewise, exchange transfusion with PolyHSA preserved vascular permeability measured with extravasation of fluorescently labeled dextran. The intravascular retention time of the exchanged PolyHSA was significantly longer compared with the intravascular retention time of HSA. Lastly, the viability of tissue subjected to ischemia-reperfusion injury increased in animals exchanged with PolyHSA compared with HSA. Therefore maintenance of microvascular perfusion, improvement in vascular integrity, and reduction in tissue damage resulting from reperfusion with PolyHSA suggest that PolyHSA is a promising fluid therapy to improve outcomes of ischemia-reperfusion injury. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
87507587
Volume :
132
Issue :
2
Database :
Complementary Index
Journal :
Journal of Applied Physiology
Publication Type :
Academic Journal
Accession number :
155849731
Full Text :
https://doi.org/10.1152/japplphysiol.00117.2021