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Characterization of micron-scale protein-depleted plasma membrane domains in phosphatidylserine-deficient yeast cells.

Authors :
Tetsuo Mioka
Tian Guo
Shiyao Wang
Takuma Tsuji
Takuma Kishimoto
Toyoshi Fujimoto
Kazuma Tanaka
Source :
Journal of Cell Science; Mar2022, Vol. 135 Issue 5, p1-15, 15p
Publication Year :
2022

Abstract

Membrane phase separation to form micron-scale domains of lipids and proteins occurs in artificial membranes; however, a similar large-scale phase separation has not been reported in the plasma membrane of the living cells. We show here that a stable micron-scale protein-depleted region is generated in the plasma membrane of yeast mutants lacking phosphatidylserine at high temperatures. We named this region the 'void zone'. Transmembrane proteins and certain peripheral membrane proteins and phospholipids are excluded from the void zone. The void zone is rich in ergosterol, and requires ergosterol and sphingolipids for its formation. Such properties are also found in the cholesterol-enriched domains of phase-separated artificial membranes, but the void zone is a novel membrane domain that requires energy and various cellular functions for its formation. The formation of the void zone indicates that the plasma membrane in living cells has the potential to undergo phase separation with certain lipid compositions. We also found that void zones were frequently in contact with vacuoles, in which a membrane domain was also formed at the contact site. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
00219533
Volume :
135
Issue :
5
Database :
Complementary Index
Journal :
Journal of Cell Science
Publication Type :
Academic Journal
Accession number :
155919935
Full Text :
https://doi.org/10.1242/jcs.256529