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Wenyang Huazhuo Tuihuang Formula Inhibits the Th17/Treg Cell Imbalance and Protects against Acute-on-Chronic Liver Failure.
- Source :
- Evidence-based Complementary & Alternative Medicine (eCAM); 3/30/2022, p1-11, 11p, 1 Color Photograph, 1 Chart, 4 Graphs
- Publication Year :
- 2022
-
Abstract
- Objective. Acute-on-chronic liver failure (ACLF) is a group of chronic liver diseases and caused by acute internal and external liver injury. Wenyang Huazhuo Tuihuang (WYHZTH) formula had a good clinical effect on promoting the resolution of jaundice. The aim of this study is to further investigate the mechanism of the WYHZTH formula in the ACLF rat model. Methods. The ACLF rat model was constructed by combining human serum albumin with LPS and D-gal. WYHZTH was used to intervene and treat. The cytokines IL-17, IL-23, IL-10, and TGF-β were detected by ELISA and fluorescence-quantitative PCR. Flow cytometry was used to detect the percentage of Th17 and Treg cells in the peripheral blood and liver tissues of each group of rats. The pathological changes in the liver tissue were detected by hematoxylin-eosin staining, immunohistochemistry, and electron microscopy. Results. Compared with the ACLF group, the WYHZTH formula and Thy significantly decreased the levels of ALT, AST, and CHE in the ACLF group. After drug intervention, apoptosis was significantly reduced. The PCNA expression decreased in the ACLF model group but increased in the WYHZTH or Thy group. Under transmission electron microscope, hepatocytes in the ACLF group showed obvious necrosis. After drug intervention, hepatocyte necrosis was reduced with most of the structure returning to normal. Conclusion. This present study demonstrated that WYHZTH formula may protect against acute-on-chronic liver failure, which may be related to the inhibition of Th17/Treg cell imbalance. [ABSTRACT FROM AUTHOR]
- Subjects :
- BIOLOGICAL models
CYTOKINES
INTERLEUKINS
TRANSFORMING growth factors-beta
FLOW cytometry
HERBAL medicine
STAINS & staining (Microscopy)
ANIMAL experimentation
IMMUNOHISTOCHEMISTRY
CELL physiology
APOPTOSIS
RATS
ELECTRON microscopy
ENZYME-linked immunosorbent assay
CHOLINESTERASES
T cells
POLYMERASE chain reaction
LIVER cells
LIVER failure
CHINESE medicine
NECROSIS
ALANINE aminotransferase
ASPARTATE aminotransferase
Subjects
Details
- Language :
- English
- ISSN :
- 1741427X
- Database :
- Complementary Index
- Journal :
- Evidence-based Complementary & Alternative Medicine (eCAM)
- Publication Type :
- Academic Journal
- Accession number :
- 156022004
- Full Text :
- https://doi.org/10.1155/2022/5652172