Comparing on Survival Rate of Porcine and Formosan Macaque GFP-iPSC Chimera with Parthenogenetic Porcine Morula or Blastocysts.

The gene-editing (GE) is of great potential to be applied to agriculture and biomedicine. The organ waiting patients may be treated with tailored made xenografts derived from porcine blastocyst that the original key developing gene has been knockout by GE but complemented with patient's induced pluripotent stem cells (iPSC). This study used day 6 porcine morulae (M) or blastocysts (BC) from chemical activated parthenogenesis and each embryo was microinjected with 10 to 15 Formosan monkey or porcine GFP-iPSCs (GFP-FMiPSC or GFP-piPSC) to generate the inter- or intra-species chimera. The embryos were then cultured in PZM-5 at 38.5°C/5% CO₂ for 24 and 48 h to assess their development. Results showed that the survival rates of GFP-FMiPSC/BC (n = 61) and GFP-piPSC/BC (n = 60) were similar at 24 h (73.4 v.s 73.3%, respectively) yet significantly different at 48 h (69.2 ± 11.2 v.s. 53.3 ± 8.9%, respectively) (P < 0.01). On day 6, M might be over aging developed and appeared less viability and fewer developed rate (7.3 and 7.4%) to BC. [ABSTRACT FROM AUTHOR]