1H-filtered correlation experiments for assignment and determination of coupling constants in backbone labelled proteins.

The implementation of [¹³Cα,¹³C′,¹⁵N,²Hα] labelled amino acids into proteins allows the acquisition of high resolution triple resonance experiments. We present for the first time resonance assignments facilitated by this new labelling strategy. The absence of ¹J_Cα,Cβ couplings enables us to measure ¹J_Cα,C′ scalar and ¹D_Cα,C′ residual dipolar coupling constants using modified HNCA experiments which do not suffer from sensitivity losses characteristic for ¹³C constant time experiments. [ABSTRACT FROM AUTHOR]