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CD45RB Glycosylation and Ig Isotype Define Maturation of Functionally Distinct B Cell Subsets in Human Peripheral Blood.

Authors :
Koers, Jana
Pollastro, Sabrina
Tol, Simon
Pico-Knijnenburg, Ingrid
Derksen, Ninotska I. L.
van Schouwenburg, Pauline A.
van der Burg, Mirjam
van Ham, S. Marieke
Rispens, Theo
Source :
Frontiers in Immunology; 4/28/2022, Vol. 13, p1-8, 8p
Publication Year :
2022

Abstract

Glycosylation of CD45RB (RB+) has recently been identified to mark antigen-experienced B cells, independent of their CD27 expression. By using a novel combination of markers including CD45RB glycosylation, CD27 and IgM/IgD isotype expression we segregated human peripheral blood B cell subsets and investigated their IGHV repertoire and in vitro functionality. We observed distinct maturation stages for CD27-RB+ cells, defined by differential expression of non-switched Ig isotypes. CD27-RB+ cells, which only express IgM, were more matured in terms of Ig gene mutation levels and function as compared to CD27-RB+ cells that express both IgM and IgD or cells that were CD27-RB-. Moreover, CD27-RB+IgM+ cells already showed remarkable rigidity in IgM isotype commitment, different from CD27-RB+IgMD+ and CD27-RB- cells that still demonstrated great plasticity in B cell fate decision. Thus, glycosylation of CD45RB is indicative for antigen-primed B cells, which are, dependent on the Ig isotype, functionally distinct. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
16643224
Volume :
13
Database :
Complementary Index
Journal :
Frontiers in Immunology
Publication Type :
Academic Journal
Accession number :
156582872
Full Text :
https://doi.org/10.3389/fimmu.2022.891316