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Pattern of lectin binding to murine T lymphocytes.

Authors :
Sowalsky, R. A.
Fox, B. S.
Source :
Immunology; Jan92, Vol. 75 Issue 1, p92-98, 7p
Publication Year :
1992

Abstract

Lectins can be used to detect changes in cell-surface glycosylation. In this paper we examine the lectin-binding characteristics of murine T cells as measured by flow cytometry. A large panel of labelled lectins was used to stain naive, activated and resting murine T cells. Some lectins did not detectably bind any T cells, some bound to all T cells and some bound to only a subset of splenic T cells. Three lectins which preferentially bind to previously activated T cells were identified: Bandeiraea simplicifolia BS-I, Bauhinia purpurea and Lycopersicon esculentum. In addition, two lectins were found to bind preferentially to activated T cells; Glycine max and Triticum vulgaris. Finally, no differences were found in the ability of a large panel of lectins to bind to Th1 and Th2 clones. Lectin binding may be a powerful tool for distinguishing naive, activated and memory T cells. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
00192805
Volume :
75
Issue :
1
Database :
Complementary Index
Journal :
Immunology
Publication Type :
Academic Journal
Accession number :
15674490