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Age-related capacity of neuroendocrine factors to differentiate T cells <em>in vitro</em>.
- Source :
- Immunology; Jan92, Vol. 75 Issue 1, p99-102, 4p
- Publication Year :
- 1992
-
Abstract
- The present report shows the capacity of hypothalamic extract (HE) to differentiate bone marrow cells to Thy-1<superscript>+</superscript> cells in vitro. A two-step short-term culture was used. In the first step thymus and pituitary were co-cultured in the presence of HE. Supernatant was then transferred to a bone marrow cell suspension and following a period of culture. The percentage of Thy-1<superscript>+</superscript> cells was determined by a microcytotoxicity assay. Results indicated that: (a) HE from young mice show a very efficient differentiating capacity; (b) HE from young mice is equally efficient when old pituitary, thymus and marrow are used; (c) HE from old donors has no capacity to differentiate T cells; (d) there is a progressive age-related decline of this capacity; and (e) there is a feed-back mechanism involved in this process. It is concluded that hypothalamic factors can regulate the differentiation of T cells and that this effect operates through a mechanism involving pituitary and thymus. [ABSTRACT FROM AUTHOR]
- Subjects :
- BONE marrow
ENDOCRINE glands
IMMUNE system
LYMPHOCYTES
T cells
CELL suspensions
Subjects
Details
- Language :
- English
- ISSN :
- 00192805
- Volume :
- 75
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 15674495