Back to Search
Start Over
Fine mapping of Pm58 from Aegilops tauschii conferring powdery mildew resistance.
- Source :
- Theoretical & Applied Genetics; May2022, Vol. 135 Issue 5, p1657-1669, 13p
- Publication Year :
- 2022
-
Abstract
- Key message: The powdery mildew resistance gene Pm58 was traced to a 141.3-kb interval with the co-segregating marker Xkasp68500 in wheat breeding. Pm58 is a powdery mildew resistance gene identified in Aegilops tauschii accession TA1662 and effective in a common wheat background. To finely map Pm58, an F<subscript>2</subscript> population of 676 plants derived from the cross T093 × TA1662 was used for recombinant screening. We obtained 13 recombinants that occurred between the flanking markers Xhnu670 and Xhnu186. Genotyping and phenotyping these recombinant F<subscript>2:3</subscript> families delimited Pm58 to a 0.22-cM interval (Xsts20220–Xkasp61553) on chromosome arm 2DS. The region carrying the Pm58 locus was approximately 141.3-kb, which contained eight annotated genes according to the reference genome sequence of Ae. tauschii AL8/78. Haplotype analysis of 178 Ae. tauschii accessions using the candidate gene-specific markers identified a disease resistance gene AET2Gv20068500 as a candidate for Pm58. Comparative mapping of the Pm58-containing interval revealed two presence/absence variations (PAVs) between AL8/78 and common wheat Chinese Spring. PAV-1 resides in the 3′-end of AET2Gv20068500. The majority of 158 common wheat cultivars (84.8%) displayed the absence of a 14.1-kb fragment in the PAV-1 region, which was confirmed by aligning the targeted genome sequences of the other sequenced Ae. tauschii accessions and common wheat cultivars. A co-segregating marker Xkasp68500 developed from AET2Gv20068500 can distinguish TA1662 from all randomly selected common wheat cultivars and will be instrumental for tracking Pm58 in breeding programs. [ABSTRACT FROM AUTHOR]
- Subjects :
- POWDERY mildew diseases
AEGILOPS
HAPLOTYPES
PLANT populations
NATURAL immunity
WHEAT
Subjects
Details
- Language :
- English
- ISSN :
- 00405752
- Volume :
- 135
- Issue :
- 5
- Database :
- Complementary Index
- Journal :
- Theoretical & Applied Genetics
- Publication Type :
- Academic Journal
- Accession number :
- 156890572
- Full Text :
- https://doi.org/10.1007/s00122-022-04061-8