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Alterations in Toll-Like Receptor 7 and -9 mRNA Levels in Lungs after Ovariohysterectomy in a Pyometra Mouse Model.
- Source :
- European Surgical Research; 2022, Vol. 63 Issue 2, p85-97, 13p
- Publication Year :
- 2022
-
Abstract
- Background: Pyometra (P) leads to sepsis and multiple organ dysfunction syndrome. Toll-like receptors (TLRs) recognize pathogens which can cause P. The aim of this study was to investigate TLR-7 and -9 via the MYD88 pathway and the nuclear factor kappa B (NFκB) response in the uterus of a P mouse model before and after ovariohysterectomy (RP) as well as potential lung injury. Materials and Methods: 200 female C57BL/6J mice were randomly divided into groups (N = 10/subgroup; sham 1, 2, 3, 7; P1, 2, 3, 7; 1RP1, 2, 3, 7; 2RP1, 2, 3, 7; 3RP1, 2, 3, 7) according to the day of euthanasia. Pathogens were administrated in the groups P and RP in order to induce P. Results: Alterations in blood chemistry, histopathology, and RT-qPCT analysis before (P) and after RP were observed. Significant correlations were also found between MYD88, NFκB, and TLR9 in P and RP groups in the lungs and in RP groups in the uterus, suggesting that the immune system responded via the TLR9-MYD88 pathway. Conclusions: This is the first report of immunohistochemical TLR-7 and -9 localization and of TLR-7, -9, MYD88, and NFκB mRNA expression in the uterus causing lung injury in a P mouse model. [ABSTRACT FROM AUTHOR]
- Subjects :
- TOLL-like receptors
NF-kappa B
LABORATORY mice
PYOMETRA
ANIMAL disease models
Subjects
Details
- Language :
- English
- ISSN :
- 0014312X
- Volume :
- 63
- Issue :
- 2
- Database :
- Complementary Index
- Journal :
- European Surgical Research
- Publication Type :
- Academic Journal
- Accession number :
- 157229323
- Full Text :
- https://doi.org/10.1159/000519425