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Cytokinin oxidase/dehydrogenase family genes exhibit functional divergence and overlap in rice growth and development, especially in control of tillering.
- Source :
- Journal of Experimental Botany; 6/2/2022, Vol. 73 Issue 11, p3552-3568, 17p
- Publication Year :
- 2022
-
Abstract
- Cytokinins play key roles in plant growth and development, and hence their biosynthesis and degradation have been extensively studied. Cytokinin oxidase/dehydrogenases (CKXs) are a group of enzymes that regulate oxidative cleavage to maintain cytokinin homeostasis. In rice, 11 CKX genes have been identified to date; however, most of their functions remain unknown. In this study, we comprehensively examined the expression patterns and functions of the CKX s in rice by using CRISPR/Cas9 technology to construct mutants of all 11 genes. The results revealed that the ckx single-mutants and higher-order ckx4 ckx9 mutant lines showed functional overlaps and sub-functionalization. Notably, the ckx1 ckx2 and ckx4 ckx9 double-mutants displayed contrasting phenotypic changes in tiller number and panicle size compared to the wild-type. In addition, we identified several genes with significantly altered expression in both the ckx4 and ckx9 single-mutant and double-mutant plants. Many of the differentially expressed genes were found to be associated with auxin and cytokinin pathways, and cytokinins in the ckx4 ckx9 double-mutant were increased compared to the wild-type. Taken together, our findings provide new insights into the functions of CKX genes in rice growth and may provide the foundations for future studies aimed at improving rice yield. [ABSTRACT FROM AUTHOR]
- Subjects :
- GENE families
RICE
PHENOTYPIC plasticity
CYTOKININS
PLANT growth
Subjects
Details
- Language :
- English
- ISSN :
- 00220957
- Volume :
- 73
- Issue :
- 11
- Database :
- Complementary Index
- Journal :
- Journal of Experimental Botany
- Publication Type :
- Academic Journal
- Accession number :
- 157279885
- Full Text :
- https://doi.org/10.1093/jxb/erac088