Semiquantitative Immunoblot Detection of 70 kDa Stress Proteins in the Carp Cyprinus carpio.

The article discusses the method of semiquantitative detection of intracellular stress proteins found in carp fish using the immunoblot technique. Stress proteins are heat shock proteins or chaperonins divided according to their molecular weight. They are considered useful biomarkers for monitoring pollution because they can withstand any environmental stress. Carp fishes were placed in an aquarium with filtered tap water for two weeks. They were then induced with heat shock proteins, but only the white muscle of the fish was used. Immunoblotting was interfered by supernatan matrix. It was concluded that immunoblotting is a suitable method for detection and determination of stress proteins.