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Improved and Flexible HDR Editing by Targeting Introns in iPSCs.
- Source :
- Stem Cell Reviews & Reports; Jun2022, Vol. 18 Issue 5, p1822-1833, 12p
- Publication Year :
- 2022
-
Abstract
- Highly efficient gene knockout (KO) editing of CRISPR–Cas9 has been achieved in iPSCs, whereas homology-directed repair (HDR)-mediated precise gene knock-in (KI) and high-level expression are still bottlenecks for the clinical applications of iPSCs. Here, we developed a novel editing strategy that targets introns. By targeting the intron before the stop codon, this approach tolerates reading frameshift mutations caused by nonhomologous end-joining (NHEJ)-mediated indels, thereby maintaining gene integrity without damaging the non-HDR-edited allele. Furthermore, to increase the flexibility and screen for the best intron-targeting sgRNA, we designed an HDR donor with an artificial intron in place of the endogenous intron. The presence of artificial introns, particularly an intron that carries an enhancer element, significantly increased the reporter expression levels in iPSCs compared to the intron-deleted control. In addition, a combination of the small molecules M3814 and trichostatin A (TSA) significantly improves HDR efficiency by inhibiting NHEJ. These results should find applications in gene therapy and basic research, such as creating reporter cell lines. [ABSTRACT FROM AUTHOR]
- Subjects :
- SMALL molecules
FRAMESHIFT mutation
GENE knockout
INTRONS
GENE therapy
CRISPRS
Subjects
Details
- Language :
- English
- ISSN :
- 15508943
- Volume :
- 18
- Issue :
- 5
- Database :
- Complementary Index
- Journal :
- Stem Cell Reviews & Reports
- Publication Type :
- Academic Journal
- Accession number :
- 157544956
- Full Text :
- https://doi.org/10.1007/s12015-022-10331-1