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High and Sustained Ex Vivo Frequency but Altered Phenotype of SARS-CoV-2-Specific CD4 + T-Cells in an Anti-CD20-Treated Patient with Prolonged COVID-19.
- Source :
- Viruses (1999-4915); Jun2022, Vol. 14 Issue 6, pN.PAG-N.PAG, 16p
- Publication Year :
- 2022
-
Abstract
- Here, we longitudinally assessed the ex vivo frequency and phenotype of SARS-CoV-2 membrane protein (aa145–164) epitope-specific CD4<superscript>+</superscript> T-cells of an anti-CD20-treated patient with prolonged viral positivity in direct comparison to an immunocompetent patient through an MHC class II DRB1*11:01 Tetramer analysis. We detected a high and stable SARS-CoV-2 membrane-specific CD4<superscript>+</superscript> T-cell response in both patients, with higher frequencies of virus-specific CD4<superscript>+</superscript> T-cells in the B-cell-depleted patient. However, we found an altered virus-specific CD4<superscript>+</superscript> T-cell memory phenotype in the B-cell-depleted patient that was skewed towards late differentiated memory T-cells, as well as reduced frequencies of SARS-CoV-2-specific CD4<superscript>+</superscript> T-cells with CD45RA<superscript>−</superscript> CXCR5<superscript>+</superscript> PD-1<superscript>+</superscript> circulating T follicular helper cell (cT<subscript>FH</subscript>) phenotype. Furthermore, we observed a delayed contraction of CD127<superscript>−</superscript> virus-specific effector cells. The expression of the co-inhibitory receptors TIGIT and LAG-3 fluctuated on the virus-specific CD4<superscript>+</superscript> T-cells of the patient, but were associated with the inflammation markers IL-6 and CRP. Our findings indicate that, despite B-cell depletion and a lack of B-cell—T-cell interaction, a robust virus-specific CD4<superscript>+</superscript> T-cell response can be primed that helps to control the viral replication, but which is not sufficient to fully abrogate the infection. [ABSTRACT FROM AUTHOR]
- Subjects :
- COVID-19
T helper cells
T cells
CD4 antigen
IMMUNOLOGIC memory
MEMBRANE proteins
Subjects
Details
- Language :
- English
- ISSN :
- 19994915
- Volume :
- 14
- Issue :
- 6
- Database :
- Complementary Index
- Journal :
- Viruses (1999-4915)
- Publication Type :
- Academic Journal
- Accession number :
- 157824026
- Full Text :
- https://doi.org/10.3390/v14061265