Characterization of a Wheat- Dasypyrum breviaristatum Chromosome Addition and Its Derived Progenies Carrying Novel Dasypyrum-Specific Gliadin Genes.

The construction of the 28-chromosome karyotype of Dasypyrum breviaristatum was undertaken using multicolor non-denaturing fluorescent in situ hybridization (ND-FISH) and Oligo-FISH painting protocols. A novel wheat-D. breviaristatum line D2138 contained 44 chromosomes including a pair of D. breviaristatum 6V^bS.2V^bL translocation chromosomes. Individual F₂ and F₃ progenies of a cross between D2138 with wheat lines CM62, MY11 and JM22, respectively, were characterized using ND-FISH and molecular markers. A relatively high chromosome alteration rate within wheat and D. breviaristatum 6V^bS and 2V^bL was observed in the three progeny populations, suggesting that chromosome 6V^bS.2V^bL has a gametocidal-like gene. The different types of translocation and deletion lines allowed localization of D. breviaristatum-specific gliadin coding genes on sub-telomeric regions of 6V^bS by PCR and acid polyacrylamide gel electrophoresis analysis. The positive effect of the D. breviaristatum 6V^bS on agronomic and quality characters was also demonstrated. The new wheat-D. breviaristatum 6V^bS and 2V^bL translocation lines will be useful as novel germplasm for breeding purposes. [ABSTRACT FROM AUTHOR]