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Signal-On and Highly Sensitive Electrochemiluminescence Biosensor for Hydrogen Sulfide in Joint Fluid Based on Silver-Ion-Mediated Base Pairs and Hybridization Chain Reaction.

Authors :
Chen, Zhonghui
Chen, Guoli
Lin, Wei
Li, Jinqiu
Fang, Lishan
Wang, Xinyang
Zhang, Ying
Chen, Yu
Lin, Zhenyu
Source :
Chemosensors; Jul2022, Vol. 10 Issue 7, pN.PAG-N.PAG, 11p
Publication Year :
2022

Abstract

Hydrogen sulfide (H<subscript>2</subscript>S) in joint fluid acts as a signal molecule to regulate joint inflammation. Direct detection of H<subscript>2</subscript>S in joint fluid is of great significance for the diagnosis and treatment of arthritis. However, due to the low volume of joint fluid and low H<subscript>2</subscript>S concentration, existing methods face the problem of the insufficient limit of detection. In this study, a highly sensitive biosensor was proposed by designing a primer probe and combining it with hybrid chain reaction (HCR) under the strong interaction between metal ions and H<subscript>2</subscript>S to achieve H<subscript>2</subscript>S detection. The primer probe containing multiple cytosine (C) sequences was fixed on a gold electrode, and the C–Ag–C hairpin structure was formed under the action of Ag<superscript>+</superscript>. In the presence of H<subscript>2</subscript>S, it can combine with Ag<superscript>+</superscript> in the hairpin structure to form Ag<subscript>2</subscript>S, which leads to the opening of the hairpin structure and triggers the hybridization chain reaction (HCR) with another two hairpin structures (H1 and H2). A large number of double-stranded nucleic acid structures can be obtained on the electrode surface. Finally, Ru(phen)<subscript>3</subscript><superscript>2+</superscript> can be embedded into the double chain structure to generate the electrochemiluminescence (ECL) signal. The linear response of the H<subscript>2</subscript>S biosensor ranged from 0.1000 to 1500 nM, and the limit of detection concentration of H<subscript>2</subscript>S was 0.0398 nM. The developed biosensor was successfully used to determine H<subscript>2</subscript>S in joint fluid. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
22279040
Volume :
10
Issue :
7
Database :
Complementary Index
Journal :
Chemosensors
Publication Type :
Academic Journal
Accession number :
158212453
Full Text :
https://doi.org/10.3390/chemosensors10070250