Back to Search
Start Over
Raoultibacter phocaeensis sp. nov., A New Bacterium Isolated from a Patient with Recurrent Clostridioides difficile Infection.
- Source :
- Current Microbiology; Sep2022, Vol. 79 Issue 9, p1-12, 12p
- Publication Year :
- 2022
-
Abstract
- Strain Marseille-P8396<superscript>T</superscript> is a new species isolated from a patient with recurrent Clostridioides difficile infection. Its optimal growth condition was observed at pH of 7.5, at a temperature of 37 °C after 72 h of incubation on Columbia agar (BioMérieux, France) with 5% sheep blood, under an anaerobic atmosphere. Strain Marseille-P8396<superscript>T</superscript> cells are Gram-positive rods, nonspore‐forming, and nonmotile. 9-Octadecenoic acid (41.9%), hexadecanoic acid (22.5%), and 11-Octadecenoic acid (11.0%) represent the major fatty acid of strain Marseille-P8396<superscript>T</superscript>. The optimal growth condition of strain Marseille-P8396<superscript>T</superscript> was observed at 37 °C after 72 h of incubation under an anaerobic atmosphere, pH ranging from 6.5 to 8.5, and salinity of 0.5 to 7.5%. Its genome (Genbank Accession Number NZ_CABDUX000000000) size was 3.86 Mb with 59.4 mol% of G+C content, and 3,124 protein-coding genes. The 16S rRNA gene sequence (Genbank accession number NR_148574.1) of strain Marseille-P8396<superscript>T</superscript> shared a similarity of 98.71% with Raoultibacter timonensis strain Marseille-P3277<superscript>T</superscript> (Genbank accession number NR_148574.1), currently the most closely related species. However, the OrthoANI and digital DNA–DNA hybridization values with Raoultibacter timonensis strain Marseille-P3277<superscript>T</superscript> (Genbank accession number OEPT01000000) were 80.15% and 24.6 ± 4.8%, respectively. Taken together, these results clearly demonstrate that strain Marseille-P8396<superscript>T</superscript> represents a new species within the genus Raoultibacter described here as Raoultibacter phocaeensis sp. nov. (type strain: Marseille-P8396<superscript>T</superscript>=CSUR8396<superscript>T</superscript>=CECT 30202<superscript>T</superscript>). [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 03438651
- Volume :
- 79
- Issue :
- 9
- Database :
- Complementary Index
- Journal :
- Current Microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 158660824
- Full Text :
- https://doi.org/10.1007/s00284-022-02959-6