Polyphenols from Thinned Young Apples: HPLC-HRMS Profile and Evaluation of Their Anti-Oxidant and Anti-Inflammatory Activities by Proteomic Studies.

Hence, based on this new vision of the antioxidant activity of natural antioxidants, as a next step, we tested the ability of TAP to act as an indirect antioxidant by activating the NRF2 pathway. Based on this overview, the major mechanism of action for nutritional antioxidants, and in particular, for plant polyphenols such as those contained in TAP, is the paradoxical oxidative activation of the NRF2 (NF-E2-related factor 2) signaling pathway, which maintains protective oxidoreductases and their nucleophilic substrates. Keywords: thinned apples; polyphenols; anti-oxidant; anti-inflammatory; NRF2; NF- B; proteomics EN thinned apples polyphenols anti-oxidant anti-inflammatory NRF2 NF- B proteomics N.PAG N.PAG 27 08/29/22 20220801 NES 220801 1. Thinned apples, polyphenols, anti-oxidant, anti-inflammatory, NRF2, NF- B, proteomics Activation of NRF2 by TAP can be firstly ascribed to the polyphenol compounds bearing an ortho-diphenol moiety which is oxidized to the corresponding quinone, which, being an electrophilic compound, reacts with the thiols of KEAP1, thus releasing NRF2, which then translocates into the nucleus. [Extracted from the article]