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Functional Analysis of B--L (la-Like) Antigen- Bearing Chicken Peripheral Blood Cells.
- Source :
- Scandinavian Journal of Immunology; Jul1984, Vol. 20 Issue 1, p15-19, 5p
- Publication Year :
- 1984
-
Abstract
- The function of B-L (Ia-equivalent)-positive (B-L<superscript>+</superscript>) and -negative (B-L<superscript>-</superscript>) chicken peripheral blood lymphocytes (PBL) was studied in vitro and in vivo. The PBL were first stained in direct immunofluorescence tests with a fluorescein isothiocyanate-labelled anti-B-L alloantiserum and then separated by means of a fluorescence-activated cell sorter. In agreement with our previous findings, B-L<superscript>-</superscript> cells showed functional properties of T lymphocytes, responding to concanavalin A and phytohaemagglutinin-P in vitro and inducing a graft-versus-host (GVH) reaction when injected into allogeneic embryos. Sorted B-L<superscript>+</superscript>" gave no responses in any of these assays. Neither B-L<superscript>+</superscript> nor B-L<superscript>-</superscript> cells, when tested alone, responded significantly to pokeweed mitogen, but mixtures of the two restored the responsiveness to that of the original unsorted suspension. Of the B-L<superscript>+</superscript> PBL, 10% were T cells, which may account for the low GVH reactivity given by this population. [ABSTRACT FROM AUTHOR]
- Subjects :
- LYMPHOCYTES
CELLS
LEUCOCYTES
FLUORESCENCE
T cells
IMMUNOCYTOCHEMISTRY
IMMUNOCHEMISTRY
Subjects
Details
- Language :
- English
- ISSN :
- 03009475
- Volume :
- 20
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- Scandinavian Journal of Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 15873721
- Full Text :
- https://doi.org/10.1111/j.1365-3083.1984.tb00972.x