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MicroRNA-143 inhibits proliferation and migration of prostate cancer cells.
- Source :
- Archives of Physiology & Biochemistry; Oct2022, Vol. 128 Issue 5, p1323-1329, 7p
- Publication Year :
- 2022
-
Abstract
- Background: Prostate cancer (PC) is one of the most prevalent types of malignancies in males. Here, we replaced the miRNA-143 in PC cells by using a vector-based miRNA-143 transfection approach. Materials and methods: The miRNA-143 vector was transfected into the cells and qRT-PCR was applied to assess the expression of target genes in PC3 cells. Also, the MTT, scratch wound-healing, and DAPI staining assays were done to assess the proliferation, migration, and apoptosis of the cells, respectively. Results: The findings of the qRT-PCR determined the enhanced expression of miRNA-143 and other cancer-associated genes. The MTT and wound-healing assays revealed the proliferation and migration reduction in the transfected cells in comparison to control cells that contain an empty vector. Conclusion: The miRNA-143 has a significant impact on cell growth and migration during PC metastasis, and it may be a promising candidate for molecular therapies of PC. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 13813455
- Volume :
- 128
- Issue :
- 5
- Database :
- Complementary Index
- Journal :
- Archives of Physiology & Biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 159297435
- Full Text :
- https://doi.org/10.1080/13813455.2020.1769678