Insight into the differences in the toxicity mechanisms of dinotefuran enantiomers in zebrafish by UPLC-Q/TOF–MS.

Dinotefuran is a chiral insecticide widely used to control Nilaparvata lugens in agriculture. However, little is known about the toxic effects of dinotefuran enantiomers on aquatic organisms. In this study, zebrafish were exposed to 1.00 and 10.00 mg/L dinotefuran enantiomers for 96 h, after which multivariate pattern recognition, metabolite identification, and pathway analysis were performed. Principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA) were then conducted to reveal the metabolic perturbations caused by dinotefuran enantiomers. Metabolic pathway analysis revealed the perturbation of five main pathways, including phenylalanine, tyrosine and tryptophan biosynthesis; phenylalanine metabolism; retinol metabolism; arginine and proline metabolism; and glycerophospholipid metabolism. These disturbed metabolic pathways were strongly correlated with energy, amino acid metabolism, and lipid metabolism. Pathway analysis also indicated that the metabolic pathway changes induced by the same level of R and S-dinotefuran were enantioselective. Our research may provide better insight into the risk of chiral dinotefuran in aquatic organisms in the environment. [ABSTRACT FROM AUTHOR]