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Changes in intestinal homeostasis and immunity in a cigarette smoke- and LPS-induced murine model for COPD: the lung-gut axis.

Authors :
Lei Wang
Pelgrim, Charlotte E.
Peralta Marzal, Lucía N.
Korver, Stephanie
van Ark, Ingrid
Leusink-Muis, Thea
van Helvoort, Ardy
Keshavarzian, Ali
Kraneveld, Aletta D.
Garssen, Johan
Henricks, Paul A. J.
Folkerts, Gert
Braber, Saskia
Source :
American Journal of Physiology: Lung Cellular & Molecular Physiology; Sep2022, Vol. 323 Issue 3, p266-280, 15p
Publication Year :
2022

Abstract

Chronic obstructive pulmonary disease (COPD) is often associated with intestinal comorbidities. In this study, changes in intestinal homeostasis and immunity in a cigarette smoke (CS)- and lipopolysaccharide (LPS)-induced COPD model were investigated. Mice were exposed to cigarette smoke or air for 72 days, except days 42, 52, and 62 on which the mice were treated with saline or LPS via intratracheal instillation. Cigarette smoke exposure increased the airway inflammatory cell numbers, mucus production, and different inflammatory mediators, including C-reactive protein (CRP) and keratinocyte-derived chemokine (KC), in bronchoalveolar lavage (BAL) fluid and serum. LPS did not further impact airway inflammatory cell numbers or mucus production but decreased inflammatory mediator levels in BAL fluid. T helper (Th) 1 cells were enhanced in the spleen after cigarette smoke exposure; however, in combination with LPS, cigarette exposure caused an increase in Th1 and Th2 cells. Histomorphological changes were observed in the proximal small intestine after cigarette smoke exposure, and addition of LPS had no effect. Cigarette smoke activated the intestinal immune network for IgA production in the distal small intestine that was associated with increased fecal sIgA levels and enlargement of Peyer's patches. Cigarette smoke plus LPS decreased fecal sIgA levels and the size of Peyer's patches. In conclusion, cigarette smoke with or without LPS affects intestinal health as observed by changes in intestinal histomorphology and immune network for IgA production. Elevated systemic mediators might play a role in the lunggut cross talk. These findings contribute to a better understanding of intestinal disorders related to COPD. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
10400605
Volume :
323
Issue :
3
Database :
Complementary Index
Journal :
American Journal of Physiology: Lung Cellular & Molecular Physiology
Publication Type :
Academic Journal
Accession number :
159393203
Full Text :
https://doi.org/10.1152/ajplung.00486.2021