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Paeoniflorin Protects H9c2 Cardiomyocytes against Hypoxia/Reoxygenation Induced Injury via Regulating the AMPK/Nrf2 Signaling Pathway.
- Source :
- Evidence-based Complementary & Alternative Medicine (eCAM); 10/13/2022, Vol. 2022, p1-8, 8p, 5 Graphs
- Publication Year :
- 2022
-
Abstract
- Myocardial ischemia/reperfusion (MIR) injury contributes to the exacerbation of heart disease by causing cardiac arrhythmias, myocardial infarction, and even sudden death. Studies have found that paeoniflorin (PF) has a protective effect on coronary artery disease (CAD). However, the mechanism of PF in MIR has not been fully investigated. The purpose of this study was to investigate the functional role of PF in H9c2 cells subjected to hypoxia/reoxygenation (H/R). Here, PF treatment enhanced cell viability in H/R-stimulated H9c2 cells. In H9c2 cells, PF treatment reduced the formation of reactive oxygen species (ROS) induced by H/R. In H/R-stimulated H9c2 cells, PF also increased the activity of antioxidant enzymes such as superoxide dismutase and glutathione peroxidase. Furthermore, PF protected H9c2 cells against H/R-induced apoptosis, as demonstrated by increased Bcl-2 expression, decreased Bax expression, and decreased caspase-3 activity. Furthermore, PF increased the levels of p-AMPK and nuclear Nrf2 expression in response to H/R stimulation. AMPK inhibition, on the other hand, abolished the PF-mediated increase in Nrf2 signaling and the cardiac-protective effect in H9c2 cells exposed to H/R. These data suggest that PF protected H9c2 cells against H/R-induced oxidative stress and apoptosis through modulating the AMPK/Nrf2 signaling pathway. Our findings support the therapeutic potential of PF in myocardial I/R damage. [ABSTRACT FROM AUTHOR]
- Subjects :
- THERAPEUTIC use of plant extracts
HERBAL medicine
MYOCARDIUM
HEART cells
NUCLEAR factor E2 related factor
ANIMAL experimentation
GLYCOSIDES
ANTIOXIDANTS
APOPTOSIS
CELLULAR signal transduction
RATS
CELL survival
OXIDATIVE stress
REACTIVE oxygen species
CHINESE medicine
HYPOXEMIA
REPERFUSION injury
THERAPEUTICS
Subjects
Details
- Language :
- English
- ISSN :
- 1741427X
- Volume :
- 2022
- Database :
- Complementary Index
- Journal :
- Evidence-based Complementary & Alternative Medicine (eCAM)
- Publication Type :
- Academic Journal
- Accession number :
- 159754341
- Full Text :
- https://doi.org/10.1155/2022/7667770