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The Banana MaWRKY18 , MaWRKY45 , MaWRKY60 and MaWRKY70 Genes Encode Functional Transcription Factors and Display Differential Expression in Response to Defense Phytohormones.

Authors :
Garcýa-Laynes, Sergio
Herrera-Valencia, Virginia Aurora
Tamayo-Torres, Lilia Guadalupe
Limones-Briones, Verónica
Barredo-Pool, Felipe Alonso
Baas-Espinola, Fray Martin
Alpuche-Solís, Angel Gabriel
Puch-Hau, Carlos
Peraza-Echeverria, Santy
Source :
Genes; Oct2022, Vol. 13 Issue 10, pN.PAG-N.PAG, 17p
Publication Year :
2022

Abstract

WRKY transcription factors (TFs) play key roles in plant defense responses through phytohormone signaling pathways. However, their functions in tropical fruit crops, especially in banana, remain largely unknown. Several WRKY genes from the model plants rice (OsWRKY45) and Arabidopsis (AtWRKY18, AtWRKY60, AtWRKY70) have shown to be attractive TFs for engineering disease resistance. In this study, we isolated four banana cDNAs (MaWRKY18, MaWRKY45, MaWRKY60, and MaWRKY70) with homology to these rice and ArabidopsisWRKY genes. The MaWRKY cDNAs were isolated from the wild banana Musa acuminata ssp. malaccensis, which is resistant to several diseases of this crop and is a progenitor of most banana cultivars. The deduced amino acid sequences of the four MaWRKY cDNAs revealed the presence of the conserved WRKY domain of ~60 amino acids and a zinc-finger motif at the N-terminus. Based on the number of WRKY repeats and the structure of the zinc-finger motif, MaWRKY18 and MaWRKY60 belong to group II of WRKY TFs, while MaWRKY45 and MaWRKY70 are members of group III. Their corresponding proteins were located in the nuclei of onion epidermal cells and were shown to be functional TFs in yeast cells. Moreover, expression analyses revealed that the majority of these MaWRKY genes were upregulated by salicylic acid (SA) or methyl jasmonate (MeJA) phytohormones, although the expression levels were relatively higher with MeJA treatment. The fact that most of these banana WRKY genes were upregulated by SA or MeJA, which are involved in systemic acquired resistance (SAR) or induced systemic resistance (ISR), respectively, make them interesting candidates for bioengineering broad-spectrum resistance in this crop. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
20734425
Volume :
13
Issue :
10
Database :
Complementary Index
Journal :
Genes
Publication Type :
Academic Journal
Accession number :
159868767
Full Text :
https://doi.org/10.3390/genes13101891