Back to Search Start Over

Structural Characterization of Peripolin and Study of Antioxidant Activity of HMG Flavonoids from Bergamot Fruit.

Authors :
Bartella, Lucia
Mazzotti, Fabio
Talarico, Ines Rosita
De Luca, Giuseppina
Santoro, Ilaria
Prejanò, Mario
Riccioni, Costanza
Marino, Tiziana
Di Donna, Leonardo
Source :
Antioxidants; Oct2022, Vol. 11 Issue 10, pN.PAG-N.PAG, 16p
Publication Year :
2022

Abstract

The structural characterization of a new flavonoid from bergamot fruit (Citrus bergamia Risso) carrying the 3-hydroxy-3-methyl glutaryl (HMG) ester moiety has been accomplished, and its antioxidant ability was tested from a chemical point of view. The peculiarity of the new molecule, named peripolin, relies on the presence of the HMG chemical group linked to the sugar portion of neoeriocitrin; the structure was elucidated using both high-resolution mass spectrometry and nuclear magnetic resonance experiments performed on the purified molecule extracted from the fruit. The antioxidant ability of the new molecule was tested by classical chemical approaches, such as DPPH, ABTS and FRAP assays, and from a theoretical point of view. <superscript>1</superscript>H and <superscript>13</superscript>C NMR experiments and HR-ESI-MS/MS experiments show unequivocally that the HMG moiety is linked to the primary position of the glucose unit of neohesperidose, while the chemical tests and the computational results show that peripolin possesses strong antioxidant behavior, similar to that of neoeriocitrin and remarkably higher respect to the other flavonoids present in the fruit. Furthermore, the quantitative assays carried out by UPLC-MS/MS showed that its amount in the fruit is similar to that of the other main flavonoids. Furthermore, molecular dynamics simulations allowed us to investigate the possible conformations adopted by the antioxidants in the presence of water molecules. In particular, the switch of open-closed conformations of HMG-containing species was evidenced. As far as the reaction with DPPH, the calculation of ΔG<subscript>rea</subscript> supported the experimental outcomes regarding the peripolin and neoeriocitrin activity. In conclusion, bergamot fruit, already known for its potential to lower the level of blood cholesterol, has been proven to contain molecules such as neoeriocitrin and the newly characterized peripolin, which could have important in-vivo antioxidant characteristics. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
20763921
Volume :
11
Issue :
10
Database :
Complementary Index
Journal :
Antioxidants
Publication Type :
Academic Journal
Accession number :
159870127
Full Text :
https://doi.org/10.3390/antiox11101847