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PotatoMASH—A Low Cost, Genome-Scanning Marker System for Use in Potato Genomics and Genetics Applications.
- Source :
- Agronomy; Oct2022, Vol. 12 Issue 10, pN.PAG-N.PAG, 24p
- Publication Year :
- 2022
-
Abstract
- We have developed PotatoMASH (Potato Multi-Allele Scanning Haplotags), a novel low-cost, genome-scanning marker platform. We designed a panel of 339 multi-allelic regions placed at 1 Mb intervals throughout the euchromatic portion of the genome. These regions were assayed using a multiplex amplicon sequencing approach, which allows for genotyping hundreds of plants at a cost of 5 EUR/sample. We applied PotatoMASH to a population of over 700 potato lines. We obtained tetraploid dosage calls for 2012 short multi-allelic haplotypes in 334 loci, which ranged from 2 to 14 different haplotypes per locus. The system was able to diagnose the presence of targeted pest-resistance markers, to detect quantitative trait loci (QTLs) by genome-wide association studies (GWAS) in a tetraploid population, and to track variation in a diploid segregating population. PotatoMASH efficiently surveys genetic variation throughout the potato genome, and can be implemented as a single low-cost genotyping platform that will allow the routine and simultaneous application of marker-assisted selection (MAS) and other genotyping applications in commercial potato breeding programmes. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 20734395
- Volume :
- 12
- Issue :
- 10
- Database :
- Complementary Index
- Journal :
- Agronomy
- Publication Type :
- Academic Journal
- Accession number :
- 159870973
- Full Text :
- https://doi.org/10.3390/agronomy12102461