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Characterization of the role of mononuclear cell subpopulations in the in vitro lymphocyte proliferation assay.
- Source :
- Clinical & Experimental Immunology; Sep1979, Vol. 37 Issue 3, p551-557, 7p
- Publication Year :
- 1979
-
Abstract
- Purified human mononuclear cell subpopulations have been evaluated in the in vitro lymphocyte proliferation assay. Monocyte-depleted mononuclear cells had reduced or absent responses to mitogens and antigens which could be restored to the original mononuclear cell response by addition of purified plastic adherent cells. Purified T lymphocytes obtained by density gradient centrifugation of E-rosette-forming cells demonstrated low but significant proliferative responses to mitogens, but no significant response to antigens. The addition of monocytes potentiated the response of purified T cells to mitogens and antigens, but did not fully reconstitute the original mononuclear cell response unless non-T lymphocytes were also present. It is concluded that mononuclear cell proliferation represents a complex mechanism of cellular interaction involving multiple subpopulations of cells. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00099104
- Volume :
- 37
- Issue :
- 3
- Database :
- Complementary Index
- Journal :
- Clinical & Experimental Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 15989062