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Single-cell transcriptomics links malignant T cells to the tumor immune landscape in cutaneous T cell lymphoma.
- Source :
- Nature Communications; 10/23/2022, Vol. 13 Issue 1, p1-18, 18p
- Publication Year :
- 2022
-
Abstract
- Cutaneous T cell lymphoma (CTCL) represents a heterogeneous group of non-Hodgkin lymphoma distinguished by the presence of clonal malignant T cells. The heterogeneity of malignant T cells and the complex tumor microenvironment remain poorly characterized. With single-cell RNA analysis and bulk whole-exome sequencing on 19 skin lesions from 15 CTCL patients, we decipher the intra-tumor and inter-lesion diversity of CTCL patients and propose a multi-step tumor evolution model. We further establish a subtyping scheme based on the molecular features of malignant T cells and their pro-tumorigenic microenvironments: the T<subscript>CyEM</subscript> group, demonstrating a cytotoxic effector memory T cell phenotype, shows more M2 macrophages infiltration, while the T<subscript>CM</subscript> group, featured by a central memory T cell phenotype and adverse patient outcome, is infiltrated by highly exhausted CD8<superscript>+</superscript> reactive T cells, B cells and Tregs with suppressive activities. Our results establish a solid basis for understanding the nature of CTCL and pave the way for future precision medicine for CTCL patients. Cutaneous T cell lymphomas (CTCL) are still poorly characterised at the molecular and single-cell level. Here, the authors analyse CTCL patient samples using single-cell RNA-seq, TCR and whole-exome sequencing, revealing the molecular profiles of malignant T cells and their association with the microenvironment and clinical outcomes. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 20411723
- Volume :
- 13
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- Nature Communications
- Publication Type :
- Academic Journal
- Accession number :
- 159896856
- Full Text :
- https://doi.org/10.1038/s41467-022-28799-3